abstract：:The calculation of within-laboratory imprecision in quality-assessment (QA) programmes normally involves combining data from different analyte concentrations to calculate an average standard deviation (SD) or coefficient of variation. However, for immunoassay neither of these parameters is concentration independent. T...

journal_title：Annals of clinical biochemistry

authors： Compton P,Cole B,Stuart M,Egan G

更新日期：1984-11-01 00:00:00

abstract：:The two stages of a two-site immunoradiometric assay were investigated separately. In the first stage, the amount of ferritin bound to coated tubes initially showed a rapid increase with increasing concentration of added ferritin. This was followed by a plateau and then a further increase which appeared to be largely ...

journal_title：Annals of clinical biochemistry

authors： Perera P,Worwood M

更新日期：1984-09-01 00:00:00

abstract：:It is our aim to summarise and discuss procedures for the evaluation of the concentration of free ionised calcium in serum or plasma. Stress is laid upon the interrelations and relative validity of the most common algebraic expressions to appraise the calcium status. The multitude of formulae proposed in the literatur...

journal_title：Annals of clinical biochemistry

authors： Vanstapel FJ,Lissens WD

更新日期：1984-09-01 00:00:00

abstract：:The levels of thyroid hormones in paired venous and capillary serum samples taken from 68 healthy full-term neonates were determined by radioimmunoassay. Compared with capillary values, total thyroxine (T4), thyrotropin (TSH) and thyroxine-binding globulin (TBG) each showed a significant decrease in venous serum. The ...

journal_title：Annals of clinical biochemistry

authors： O'Grady CM,Franklin RC

更新日期：1984-07-01 00:00:00

abstract：:The total cost of a haematology and clinical chemistry laboratory, including external and hospital costs, was studied in a district general hospital in The Netherlands. The main conclusion is that the number of samples analysed is the most important cost-setting factor, but that reduction in the number of requests doe...

journal_title：Annals of clinical biochemistry

authors： Leijten JF,van der Geer F,Scholten MN,Goldschmidt HM

更新日期：1984-03-01 00:00:00

abstract：:There is an increasing need for sensitive and specific methods for toxicological screening. Development of a dual-column gas chromatography system for the screening of drugs and metabolites in the plasma of poisoned patients is described. The system has been evaluated using plasma from a well-documented series of pati...

journal_title：Annals of clinical biochemistry

authors： Jarvie DR,Simpson D

更新日期：1984-03-01 00:00:00

abstract：:This study was undertaken to investigate the extent of the reported discrepancy between serum and plasma albumin concentrations measured by the bromocresol purple (BCP) method. We have shown that plasma albumin concentrations are falsely increased by turbidity due to the precipitation of fibrinogen when plasma is dilu...

journal_title：Annals of clinical biochemistry

authors： Hill PG,Wells TN

更新日期：1983-09-01 00:00:00

abstract：:Many techniques for the estimation of specific proteins measure light scattered by, or the turbidity of, suspensions of antigen:antibody aggregates formed in fluid-phase reactions. Although the shapes of reaction curves obtained are well documented, interpretation of the data in relation to the laws of light scatterin...

journal_title：Annals of clinical biochemistry

authors： Deverill I,Lock RJ

更新日期：1983-07-01 00:00:00

abstract：:The deproteinised sample (150 microliters) is 'injected' into a continuously flowing stream of deionised water which is pumped, via a capillary tube, to the nebuliser of an atomic absorption spectrophotometer. Analytical readout is obtained, in the form of transient peaks, 6 s after sample injection. Before injection ...

journal_title：Annals of clinical biochemistry

authors： Rocks BF,Sherwood RA,Turner ZJ,Riley C

更新日期：1983-03-01 00:00:00

abstract：:A sensitive radioimmunoassay method for sodium cromoglycate in human plasma is described. The lowest quantifiable concentration of sodium cromoglycate is 0.93 nmol/l when 0.1 ml plasma samples are analysed. Direct analysis of sodium cromoglycate concentrations in plasma samples collected up to several hours after the ...

journal_title：Annals of clinical biochemistry

authors： Brown K,Gardner JJ,Lockley WJ,Preston JR,Wilkinson DJ

更新日期：1983-01-01 00:00:00

abstract：:A total of 3227 urine specimens were analysed to investigate the applicability of a firefly luciferase assay of microbial adenosine triphosphate (ATP) for the rapid screening of bacteriuria in clinical specimens. Urine sediment, dipslide culture, and three plate cultures were also investigated in the majority of the u...

journal_title：Annals of clinical biochemistry

authors： Ruokonen A,Koskinen M,Leinonen M,Tiilikainen A,Vihko R

更新日期：1982-11-01 00:00:00

abstract：:Neuropeptides are present in brain tissue as well as in cerebrospinal fluid. Studies from a variety of disciplines have demonstrated that neuropeptides have neuromodulatory activities. Such activities are apparent in the involvement of neuropeptides in the regulation of adaptive, autonomic, and endocrine functions of ...

journal_title：Annals of clinical biochemistry

authors： Burbach JP

更新日期：1982-07-01 00:00:00

abstract：:An automated method for the determination of N-acetyl-beta-D-glucosaminidase in serum or plasma using p-nitrophenol (pNP) N-acetyl-beta-D-glucosamine as substrate and a Pye Unicam AURA system is described. Normal samples had activities of 853 +/- 146 (SD) nmol pNP liberated/ml/h, with intra-assay coefficient of variat...

journal_title：Annals of clinical biochemistry

authors： Moore JC,Morris JE

更新日期：1982-05-01 00:00:00

abstract：:An evaluation of the Amerlex system for determination of total thyroxine (T4) and total triiodothyronine (T3) is described. The within- and between-batch precisions were acceptable, and analyses of quality control material and linearity studies demonstrated good accuracy at the clinical decision levels. The correlatio...

journal_title：Annals of clinical biochemistry

authors： Wilke TJ,Turnbull PA

更新日期：1982-03-01 00:00:00

abstract：:A comparison has been made between three methods for measuring capillary blood glucose collected on filter paper, including a new method using a Beckman glucose analyser 2. The methods are suitable for use with capillary blood spots collected onto filter paper for monitoring the control of diabetic patients. Their acc...

journal_title：Annals of clinical biochemistry

authors： Taylor RP,Pennock CA

更新日期：1982-01-01 00:00:00

abstract：:A rapid and simple method for detecting circulating immune complexes based on turbidity measurements following polyethylene glycol precipitation was studied with regard to its suitability as a routine assay in clinical laboratories. This method was found to have an acceptable degree of precision provided the temperatu...

journal_title：Annals of clinical biochemistry

authors： Kilpatrick DC,Weston J,Irvine WJ

更新日期：1981-11-01 00:00:00

abstract：:Improvement in the control of diabetic patients is aided by a knowledge of blood glucose levels during a 'normal' (non-hospitalised) day. We have devised a 5 microliter capillary tube collection system as a 'kit' for home use by diabetics. Blood collected into 5 microliter capillary tubes is washed into a protein prec...

journal_title：Annals of clinical biochemistry

authors： Williams CP,Davies GK,Child DF

更新日期：1981-09-01 00:00:00

abstract：:A fast electrophoretic method for measuring glycosylated haemoglobin (HbA1) is described. Eight samples per cell can be assayed in a single run. Results are compatible with those found by macro-column cation-exchange chromatography; precision is good. The practical short-comings of the commercially ready-made mini-col...

journal_title：Annals of clinical biochemistry

authors： Thornton WE,Schellekens AP,Sanders GT

更新日期：1981-05-01 00:00:00

abstract：:A simple procedure is described for the preparation of a stable precision quality control material for use in the measurement of level of ionised calcium in serum at or near the reference range. Repeat analyses on a Nova 2 ionised calcium analyser of serum pools stored at different temperatures over a period of three ...

journal_title：Annals of clinical biochemistry

authors： Fyffe JA,Jenkins AS,Bolland CJ,Dryburgh FJ,Gardner MD

更新日期：1981-03-01 00:00:00

abstract：:Many attempt are now being made by external agencies to assess the quality of assay performance in hormone laboratories. Some of the problems associated with this are discussed and some remedies are proposed. ...

journal_title：Annals of clinical biochemistry

authors： Jeffcoate SL

更新日期：1981-01-01 00:00:00

abstract：:Adequate control of performance in immunoassays of maternal serum alpha-fetoprotein is an essential part of screening for neural tube defects. Information derived from external quality assessment schemes may be of limited value in monitoring individual laboratory performance and stresses the need for proper internal q...

journal_title：Annals of clinical biochemistry

authors： Woodhead JS,Kemp HA,Nix AB,Rowlands RJ

更新日期：1980-11-01 00:00:00

abstract：:A simple and improved method of continuous-flow analysis for total and direct bilirubin in serum is described. Advantages of the method are: small serum and reagent volumes; the use of less concentrated caffeine solution in the accelerator; the substitution of distilled water for hydrochloric acid in the direct biliru...

journal_title：Annals of clinical biochemistry

authors： Lolekha PH

更新日期：1980-09-01 00:00:00

abstract：:Lack of accuracy in expressing concentrations of specific proteins in different lots of Hyland calibrating reference and quality control materials, supplied for the Hyland PDQ laser nephelometer, prompted an attempt to find alternative and more reliable calibrating materials available commercially. Laser grade (Atlant...

journal_title：Annals of clinical biochemistry

authors： Shulman G

更新日期：1980-07-01 00:00:00

abstract：:Simultaneous localisation of the isoenzymes of alkaline phosphatase and gamma-glutamyl transferase was carried out on 173 serum samples from patients with hepatobiliary and bone disease to determine (i) if a better discrimination of the underlying pathology could be obtained by such an approach, and (ii) if the pathol...

journal_title：Annals of clinical biochemistry

authors： Baines M

更新日期：1980-01-01 00:00:00

abstract：:Immunoreactive melatonin has been studied in normal human volunteers, both men and women, at different times of day and different times of the year. At 2400, but not at 0800 or 1200, there are very large inter-individual variations in mean levels, the maximum difference being fivefold. Intra-individual variations are ...

journal_title：Annals of clinical biochemistry

authors： Arendt J,Wirz-Justice A,Bradtke J,Kornemark M

更新日期：1979-11-01 00:00:00

abstract：:In the last few years, the use of enzyme labels in immunoassays has been investigated. The aim of this review is to evaluate critically the role of such labels in clinical biochemistry. Special attention has been given to the problems involved in preparing enzyme labels and the ways in which such labels can be used in...

journal_title：Annals of clinical biochemistry

authors： O'Sullivan MJ,Bridges JW,Marks V

更新日期：1979-09-01 00:00:00

abstract：:This review describes the development and application of a novel test to determine levels of human immunoreactive trypsin, an enzyme produced solely by the pancreas, in biological fluids. Being organ-specific, the assay of immunoreactive trypsin should be an ideal marker of pancreatic function, and this is supported b...

journal_title：Annals of clinical biochemistry

authors： Stagg BH,Wood TP

更新日期：1979-05-01 00:00:00

abstract：:Eleven unfit bank executives in sedentary employment exercised daily for 10 weeks according to the Canadian Air-Force 5BX Exercise Programme. Over this period no changes were observed in the glucose or insulin responses to a standard breakfast or to intravenous glucose, or in the glucose response to intravenous insuli...

journal_title：Annals of clinical biochemistry

authors： Moore RA,Penfold WA,Simpson RD,Simpson RW,Mann JI,Turner RC

更新日期：1979-03-01 00:00:00

abstract：:A real time data processing system for a group of three clinical chemistry laboratories (one base and two satellite) has been developed as a successor to one used previously. This paper describes both the inadequacies of the previous worksheet-based system and the advantages of the new system, including flexibility, f...

journal_title：Annals of clinical biochemistry

authors： Hirst AD,Naylor D,White HL,Walter M

更新日期：1979-01-01 00:00:00

abstract：:The activity of serum deoxycytidylate deaminase, not hitherto reported to increase after myocardial infarction, has been investigated and the results have been related to other cardiac enzymes, including the MB fraction of creatine phosphokinase. Deoxycytidylate deaminase was found to increase to maximum activity on t...

journal_title：Annals of clinical biochemistry

authors： Roberts RG,Davies AG,Jones DD

更新日期：1978-11-01 00:00:00

abstract：:A comparative evaluation of Beckman (A), Boehringer (B), and Calbiochem (C) kits for the enzymatic end-point determination of urea has been carried out. Acceptable within-day and day-to-day precision was obtained with all kits. The recoveries obtained fulfilled the criteria of Logan (CRC Critical Reviews in Clinical L...

journal_title：Annals of clinical biochemistry

authors： Fraser CG,Fudge AN

更新日期：1978-09-01 00:00:00

abstract：:A simultaneous deterioration in performance of several radioimmunoassays suggested that equipment was malfunctioning. Replicate counts from an automated gamma counter showed unacceptable variation during a 24-hour period; this variation was detected using Trigg's technique, a sensitive method for trend analysis, and w...

journal_title：Annals of clinical biochemistry

authors： Challand GS,Cox L

更新日期：1978-03-01 00:00:00

abstract：:An automated assay for serum amylase is described. Using the AutoAnalyzer II the method employs, as substrate, a solution of dyed amylopectin which is commercially available (DyAmyl-L). After incubation of serum and substrate at 55 degrees C, 1.0 mol/lKOH is introduced and the coloured products of enzymic hydrolysis a...

journal_title：Annals of clinical biochemistry

authors： Carney JA,Osibeluwo SA,Clements JA,Jay RF

更新日期：1977-11-01 00:00:00

abstract：:The System 1 produced results for glucose and urea which were precise and accurate. It proved suitable as a general laboratory instrument as it samples 28 mul of specimen, is able to calibrate itself, produces results in three minutes, and has the capacity to analyse 56 samples an hour. ...

journal_title：Annals of clinical biochemistry

authors： Potezny N,Quelch J,Fuller B,Geary TD

更新日期：1977-09-01 00:00:00

abstract：:During incubation with rabbit blood in vitro rabbit-muscle lactate dehydrogenase-5 was inactivated at a rate similar to that observed in vivo. By contrast plasma and plasma containing erythrocytes had no effect on the enzyme activity, but plasma containing leucocytes inactivated the enzyme at the same rate as whole bl...

journal_title：Annals of clinical biochemistry

authors： Qureshi AR,Wilkinson JH

更新日期：1977-01-01 00:00:00

abstract：:Erythrocuprein (superoxide dismutase) has recently been shown to have an enzymic function towards superoxide anions. The discovery of superoxide dismutase, its mode of action, and estimation are reviewed along with a brief introduction to oxygen activation and free-radical chemistry. The formation, activity, and destr...

journal_title：Annals of clinical biochemistry

authors： Gutteridge JM

更新日期：1976-05-01 00:00:00

abstract：:The estimation of oestrogen in aqueous solutions and pregnancy urines using an automated procedure is affected by the presence of certain ions, and by dilution. These effects have been studied by calculating regression equations from the values obtained with urines to which ions have been added with and without urine ...

journal_title：Annals of clinical biochemistry

authors： Simkins A,Worth HG

更新日期：1976-01-01 00:00:00

abstract：:Three characteristics of a sample of AutoAnalyzer Sampler II modules have been measured: eccentricity of the cam, eccentricity of the spindle driving the cam, and the lengths of the chords of the cam lobes. Imprecision in timing may make a significant contribution to analytical error. ...

journal_title：Annals of clinical biochemistry

authors： McLelland AS,Fleck A,Wagstaff D,Robinson R

更新日期：1975-07-01 00:00:00

abstract：:In a group of 121 adult patients with end-stage chronic renal failure who had been undergoing maintenance haemodialysis for up to 10 years a number of biochemical variables have been measured and related to a set of objective radiological changes in the same patients. The changes in plasma calcium, magnesium, phosphat...

journal_title：Annals of clinical biochemistry

authors： Moorhead JF,Varghese Z,Tatler GL,Baillod RA,Wills MR

更新日期：1975-05-01 00:00:00

abstract：:A rapid gas liquid chromatography method has been developed which is suitable for emergency estimations of paracetamol in overdose. The method is compared with a standard ultraviolet method. ...

journal_title：Annals of clinical biochemistry

authors： Stewart MJ,Willis RG

更新日期：1975-01-01 00:00:00